![]() ![]() It is negatively charged at physiological pH values. The dye possesses very high extinction coefficient and good quantum yield, outstanding photostability, and high hydrophilicity due to the presence of two sulfo groups. sulfo-Cyanine3 is a water soluble and hydrophilic version of Cyanine3 fluorophore. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest. This reagent can be used to attach Cyanine3 fluorophore (an analog of C圓®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).Cyanine 3 Phosphoramidite 1-1'- propyl]-3,3,3',3'-tetramethylindocarbocyanine chloride Product Specifications Formula: C 58 H 70 N 4 O 4 PCl M.W.: 953.64 F.W.: 507.59 CAS Number: 18 To Retrieve a Catalog Number, Select a Pack Size and Format: 1.Lengthening smFRET lifetimes: We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a C圓/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. Optical density values in excess of 6 may appear noisy because. All transmission and blocking (OD) data are actual, measured spectra of representative production lots. Add some data! Use the legend to add fluorochromes, filters sets and individual filters to the plot. ![]()
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